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Introduction to Custom Monoclonal Antibody Production Service Case Studies Vol. 8

Introduction to Custom Monoclonal Antibody Production Service Case Studies Vol. 8

An article introducing the selection of antibodies that distinguish specific proteins within family proteins.

Antibody artisans produce antibodies entirely in vitro without using animal immunization, allowing for detailed antigen design, such as narrowing down sequences and confirming cross-reactivity with different antigens during selection. In this case, we will introduce the antigen setup and selection method for producing antibodies that belong to a family of target proteins, binding only to the desired protein and not to other proteins within the same family. 【Contents】 ■1. Specificity of antibodies ■2. Preparation of antigens 　・2-1. Design of antigens 　・2-2. Production of conjugates ■3. Methods of selection 　・3-1. Panning 　・3-2. Screening ELISA ■4. Key points of selection 　・4-1. Design of antigens 　・4-2. Enhancing antibody specificity ■5. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 10

Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 10

It is possible to design fine antigens! An article introducing an example of antibody production that distinguishes a single amino acid substitution.

Antibody artisans perform selection in vitro against fixed antigens without using animal immunity. As a result, it is possible to conduct binding reactions in the state or environment where peptides or proteins are prepared, allowing for detailed antigen design, such as narrowing down the area of interest. Here, we will introduce an example of producing an antibody that can distinguish a substitution of one amino acid within a specific sequence. We encourage you to read on. [Contents] 1. Preparation of Antigens - 1-1. Narrowing down the target area - 1-2. Setting up antigens including negative controls - 1-3. Synthesizing peptides 2. Selection to Increase Specificity - 2-1. Excluding antibodies that bind to ends, etc. - 2-2. Increasing selection pressure with multiple antigens 3. Selection of Antibodies - 3-1. Checking the signal-to-noise ratio with ELISA - 3-2. Confirming the nucleotide sequence of the antibody 4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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[Information] Monoclonal Antibody Production Contract Service "Antibody Craftsman"

[Information] Monoclonal Antibody Production Contract Service "Antibody Craftsman"

It is a selection of antibodies that can distinguish slight differences at the protein terminal!

This document is about the contract service for producing monoclonal antibodies called "Antibody Craftsman," which conducts all selections in vitro without using animal immunity. It allows for binding reactions to be performed with peptides or proteins in their prepared state, without processes such as fragmenting proteins for antigen presentation. As a result, it enables detailed antigen design, such as narrowing down sequences. In this instance, we will introduce the antigen setup and selection method for producing antibodies that can distinguish between fragments before and after cleavage by a certain protease. 【Contents】 ■ Specificity of the antibody ■ Design of the antigen ■ Screening methods ■ Protein antigens and peptide antigens ■ Summary *For more details, please refer to the PDF document or feel free to contact us.

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Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 9

Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 9

Not mediated by animal immunity! An article introducing successful cases of antibodies that distinguish specific oxidized Cys.

DJ-1 is a 21 kDa protein, and we present a successful case of producing antibodies that specifically recognize the oxidized form of the Cys residue at position 106 (C106) of DJ-1. We have attempted to obtain antibodies that specifically recognize the oxidized C106 through the production of polyclonal antibodies via immunization in rabbits and monoclonal antibodies via immunization in mice, but we were unable to obtain the desired antibodies in either case. Here, we introduce a method for producing antibodies that do not involve animal immunization and are selected entirely in vitro, along with the results of verifying the specificity of the obtained antibodies. 【Contents】 ■1. What is DJ-1 ■2. Strategic Screening 　・2-1. Antigen Design 　・2-2. Panning and ELISA Screening 　・2-3. Cross-reactivity Confirmation by ELISA ■3. Western Blotting and Immunoprecipitation ■4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF document or feel free to contact us.

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【Feature 1】Seven characteristics of monoclonal antibody production contract services.

【Feature 1】Seven characteristics of monoclonal antibody production contract services.

The success rate over the past three years boasts over 90% and has been adopted by many pharmaceutical companies.

1. Strategic Selection By using control antigens for strategic selection, it is possible to produce antibodies with special specificities, such as those that recognize the presence or absence of post-translational modifications, or antibodies that recognize common parts of homologous proteins. 2. Production of Antibodies Difficult to Obtain through Animal Immunization Since it does not rely on animal immunization, it is possible to produce antibodies against human or mouse antigens regardless of immune tolerance. Additionally, it is possible to produce antibodies against toxic antigens or immunosuppressive substances. Furthermore, since there is no antigen presentation step, it is also possible to produce antibodies that recognize natural three-dimensional structures. 3. Delivery of Candidate Antibodies in Just 7 Weeks Thanks to automated screening of antibody libraries, automated sequencing, and automated ELISA analysis, the time required from receipt of the antigen to delivery of candidate antibodies (up to 5) is just 7 weeks. For points 4 to 7, please refer to the seven features of the monoclonal antibody production contract service in Feature 2. *For more details, please refer to the PDF document or feel free to contact us.

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Monoclonal Antibody Production Contract Service Prices

Monoclonal Antibody Production Contract Service Prices

The price is based on standard antibody production costs. A precise estimate will be provided after consultation for each project, considering the most efficient process.

Antibody Production Pricing Standard Antibody Production = ¥1,800,000 per antigen Provision of 1 to 5 candidate antibodies (200μg each) from which the customer can select the final antibody, provided at 1mg (Myc-His tagged, ELISA positive). Additional Sequencing = ¥50,000 per 10 clones Additional sequencing for the same antigen. *The standard price includes sequencing for 10 clones. Additional Candidate Antibodies = ¥50,000 per candidate antibody Additional candidate antibodies for customer confirmation against the same antigen (200μg each). *The standard price includes delivery of up to 5 clones. Additional Final Antibody Production = ¥70,000 per 1mg Additional production of final antibodies for the same antigen simultaneously. *The standard price includes delivery of 1 clone (1mg). *For more details, please refer to the PDF document or feel free to contact us.

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Monoclonal antibody production contract option service

Monoclonal antibody production contract option service

Format conversion

Format Conversion The antibodies obtained through the Antibody Artisan's contract services come with various optional services. If an Fc region is required, we can convert the candidate antibody's Fab into full-length human, human-mouse chimeric, or human-rat chimeric forms, and deliver purified IgG molecules within 10 weeks. After cloning the genes for the VH and VL regions along with the desired constant region into a vector, we simultaneously introduce the light chain and heavy chain vectors into mammalian cells for expression. For more details, please also refer to the selection of antibody formats in the service flow. For pricing, please inquire for confirmation. *This is an optional service for antibodies produced through the Antibody Artisan service. *This menu is not applicable to antibodies you already possess. *For more details, please refer to the PDF materials or feel free to contact us.

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Monoclonal antibody production contract option service

Monoclonal antibody production contract option service

Production of two types of antibodies (sandwich pair) with different antigenic determinants (epitopes).

Production of two types of antibodies (sandwich pair) with different antigen determinants (epitopes). The antibodies obtained through the Antibody Artisan's contract services come with various optional services. The sandwich method serves as the foundation for many research and diagnostic assay systems. At Antibody Artisan, we provide methods to discover the best-matching antibody pairs. For pricing, please inquire for confirmation. *This is an optional service for antibodies produced by Antibody Artisan. *The menu is not applicable to antibodies you already possess. *For more details, please refer to the PDF materials or feel free to contact us.

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Monoclonal antibody production contract service option

Monoclonal antibody production contract service option

Improvement of affinity after antibody production

Improvement of Affinity After Antibody Production The antibodies obtained through the Antibody Artisan's contract services come with various optional services. The Antibody Artisan offers services to further enhance the affinity of the produced antibodies. In the HuCAL PLATINUM library, each CDR region can be freely replaced like a cassette, and by combining this with high-speed screening technology, it is possible to rapidly improve the affinity of antibodies by 10 to 1,000 times. Please inquire for pricing details. *This is an optional service for antibodies produced through the Antibody Artisan service. *This menu is not applicable to antibodies you already possess. *For more details, please refer to the PDF materials or feel free to contact us.

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Monoclonal antibody production contract option service

Monoclonal antibody production contract option service

Production of various tags and fusion proteins.

Production of various tags and fusion proteins The antibodies obtained through the contract services of Antibody Craftsman come with various optional services. Since the HuCAL gene is modular, it allows for a wide range of antibody formats to be selected, and it is also possible to add various tags or produce fusion proteins with different partners. Once candidate antibodies are determined, it is easy to change the Fab format or convert to another immunoglobulin isotype. For combinations of formats and tags, please refer to the product manual "Fab Antibody Format and Tag Combinations" available on the download site. For pricing, please inquire for confirmation. *These are optional services for antibodies produced through the Antibody Craftsman service. *This menu is not applicable to antibodies you already possess. *For more details, please refer to the PDF materials or feel free to contact us.

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